β-Galactosidase from Penicillium canescens. Properties and immobilization
نویسندگان
چکیده
منابع مشابه
Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization.
A novel β-galactosidase from Lactobacillus plantarum (LPG) was over-expressed in E. coli and purified via a single chromatographic step by using lowly activated IMAC (immobilized metal for affinity chromatography) supports. The pure enzyme exhibited a high hydrolytic activity of 491 IU/mL towards o-nitrophenyl β-D-galactopyranoside. This value was conserved in the presence of different divalent...
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β-galactosidase simply known as lactase is a significant enzyme in food industry mainly used to hydrolyse lactose in milk. In addition to provide lactose free milk for lactose intolerant people, hydrolysis of lactose avoids textural problems in frozen milk products. β-galactaosidase from different sources has been immobilized on many various type of matrixes including chitosan, alginate, sepabe...
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A method for immobilization of β-galactosidase from Kluyveromyces lactis on an adsorptive membrane for the continuous synthesis of galacto-oligosaccharides from lactose was carried out. The immobilization was performed at 4, 10, 15 and 40°C. Two strongly basic anion exchange membranes with tradename MustangQ and SartobindQ were investigated. In static experiments, the highest enzyme activity wa...
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A β-galactosidase from Cicer arietinum seeds has been purified to apparent electrophoretic homogeneity using a combination of various fractionation and chromatographic techniques, giving a final specific activity of 220 units mg(-1), with approximately 1840 fold purification. Analysis of the protein by SDS-PAGE revealed two subunits with molecular masses of 48 and 38 kDa, respectively. These ba...
متن کاملβ-galactosidase in larynx cancer
es, such as α-mannosidases (MAN) and β-galactosidases (GAL), are found in different glycoside hydrolase sequencebased families. Considerable research has proved plays the role of MAN, which play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer. Therefore the study aim was to investigate the activities of MAN and GAL in larynx...
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ژورنال
عنوان ژورنال: Open Chemistry
سال: 2005
ISSN: 2391-5420
DOI: 10.2478/bf02476241